[Sample Essay] Cell Transport Mechanisms and Permeability Essay

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1.Describe two variables that affect the rate of diffusion. a.Molecular weight  b.Solute concentration  2.Why do you think the urea was not able to diffuse through the 20 MWCO membrane? How well did the results compare with your prediction? a.Urea was unable to diffuse through the 20MWCO membrane because urea is a big molecule and the 20MWCO membrane pore size was too small for Urea. My predication was accurate, as I predicted that Urea would not be able to diffuse through the 20MWCO membrane

3.Describe the results of the attempts to diffuse glucose and albumin through the 200 MWCO membrane. How well did the results compare with your prediction? a.Glucose was able to diffuse through the 200MWCO membrane at a 0.0040 rate. However, albumin was not able to diffuse through the membrane as predicted. Albumin is a large protein that was not able to diffuse through the pore size of a 200MWCO membrane. 4.Put the following in order from smallest to largest molecular weight: glucose, sodium chloride, albumin, and urea.

a.Sodium Chloride, urea, glucose and albumin

Activity 2 Simulated Facilitated Diffusion

1.Explain one way in which facilitated diffusion is the same as simple diffusion and one way in which it differs. a.Both facilitated diffusion and simple diffusion move down a concentration gradient. b.Facilitated diffusion requires membrane carriers, while simple diffusion does not require it.

2.The larger value obtained when more glucose carriers were present corresponds to an increase in the rate of glucose transport. Explain why the rate increased. How well did the results compare with your prediction? a.When the glucose concentration increased, this increased the concentration difference between the two sides of the membrane. More molecules were available to pass through the membrane to reach equilibrium.

3.Explain your prediction for the effect Na+Cl− might have on glucose transport. In other words, explain why you picked the choice that you did. How well did the results compare with your prediction? a.I predicted that NaCl would not effect the glucose transport rate. I made this prediction because, NaCl was not required to transport glucose in the previous exercise and there was no indication that NaCl needed to use the same membrane carrier as glucose. So the NaCl would not interfere with glucose diffusion.

Activity 3 Simulating Osmotic Pressure

1.Explain the effect that increasing the Na+Cl− concentration had on osmotic pressure and why it has this effect. How well did the results compare with your prediction? a.When NaCl was increased on the left side, the water concentration on the left side decreased, thus, water wanted to move from the right to the left down its concentration gradient with more pressure. This lead to increase in pressure on the right side to prevent the water from crossing the membrane.

2.Describe one way in which osmosis is similar to simple diffusion and one way in which it is different. a.Both in simple diffusion and in osmosis, movement is from a higher concentration to a lower concentration. However in simple diffusion both water and solutes are able to diffuse, however in osmosis only water is able to diffuse.

3.Solutes are sometimes measured in milliosmoles. Explain the statement, “Water chases milliosmoles.” a.This statement refers to the fact that as the solute concentration increase, water concentration decreased. 4.The conditions were 9 mM albumin in the left beaker and 10 mM glucose in the right beaker with the 200 MWCO membrane in place. Explain the results. How well did the results compare with your prediction?

a.Because glucose is able to cross the membrane, equilibrium will be reached, with time both side had equal amounts of glucose. However, albumin is unable to cross the membrane, this will result in osmosis or an increase in osmotic pressure on the left to prevent water from crossing to the left side from the right. In this experiment, the osmotic pressure increased on the left side as I predicted it would.

 

 

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