Photosynthesis Spectroscope Absorption Spectrum & Pigment Chromatography

Photosynthesis Spectroscope Absorption Spectrum & Pigment Chromatography Photosynthesis Spectroscope Absorption Spectrum & Pigment Chromatography PHOTOSYNTHESIS REPORT SHEET Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format. All blue shaded areas require answers. I. Using the spectroscope Experiment (dry lab) View List colors not absorbed by the filter/extract (colors you see) Red filter Blue filter Green filter Spinach extract What is the relationship between the color of the filter and that filter’s absorption of light? Both the spinach extract and the green filter are green in color. How does the spinach extract compare to the absorption spectrum of the green filter? II. Absorption spectrum Photosynthesis Spectroscope Absorption Spectrum & Pigment Chromatography Experiment (dry lab) 400 nm 425 450 475 500 525 550 575 600 625 650 675 700 Carrot extract Spinach extract Absorption Spectrum Graph for carrot extract ( orange line ) and spinach extract ( green line ), superimposed. (Fill table using Microsoft insert shapes or manually graph, scan, embed image into report.). CCCC Photosynthesis Spectroscope Absorption Spectrum & Pigment Chromatography Lab Report What wavelengths and colors of light are not absorbed well (reflected/transmitted) by the carrot extract (carotenoids)? What wavelength and colors of light is not absorbed well (reflected/transmitted) by the spinach extract (chlorophylls mainly)? III. Pigment chromatography ORDER NOW FOR CUSTOMIZED AND ORIGINAL NURSING PAPERS Experiment (dry lab) Which pigment has the greatest affinity for the solvent (is most soluble in the solvent and travels right along with the solvent)? Which pigment has the greatest affinity for the paper (is most attracted to the paper)? Distance traveled by the solvent in cm 54 mm = 5.4 cm Pigment color Distance traveled in cm (from baseline to pigment band) *Rf value (pigment distance divided by solvent distance) carotenes Orange 4.8 cm 4.8/5.4 = 0.89 xanthophylls chlorophyll a chlorophyll b What pigment do you think is present in the highest concentration during the spring season when many leaves of deciduous plants are green? In the fall, leaves turn red, orange, yellow, and eventually brown. Where did the green color go? IV. Starch localization Experiment (dry lab) What would be your hypothesis on the localization of starch? Does this correspond to the localization of chlorophyll pigments? Which part of the leaf stores starch? (darker outer region or lighter inner region) V. Chloroplast structure Experiment (dry lab) Match the descriptions to the labels below a.site for light reactions, electron transport chain and ATP-synthesizing enzymes b.Site for Calvin cycle c.Surrounds the organelle Thylakoid Stroma Outer membrane CELLULAR RESPIRATION REPORT SHEET Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format. All blue shaded areas require answers. Oxygen Consumption Experiment (dry lab) Organism type (common name) Organism mass (g) Trial No. Volume O 2 used (ml) Time interval (seconds) Time interval (hour) ml O 2 / g / hr 1 2ml 150 0.042 2.11 2 2ml 180 3 2ml 175 average 2ml Plot a graph relating metabolic rate and mass. Animal Metabolic rate(ml O2/g/hr) Approximate mass Kangaroo mouse 1.8 0.02 kg Rat 0.87 1 kg Cat 0.68 2 kg Dog 0.33 7 kg Man 0.21 70 kg Elephant 0.07 1000 kg (Fill table using Microsoft insert shapes or manually graph, scan, embed image into report.) As body mass increases, oxygen consumption ____. II. CO 2 production in plant embryos Experiment (dry lab) seeds Phenol redcolor at 0 min (initial color) Phenol red color at 60 min (red, yellow, or fuchsia) Was there a change in pH? (yes or no) Is CO 2 present or absent? Bottle #1: (GERM) Red Bottle #2: (GERM – Boil) Red Experiment (at home lab) Color of 5 ml of cabbage juice Color of 5 ml of cabbage juice after blowing CO2 bubbles seeds Cabbage juice color at 0 min Cabbage juice color at 60 min (lighter, darker or same color) Was there a change in pH? (yes or no) Is CO 2 present or absent? Jar #1: germinating ( boiled ) Violet Jar #2: germinating ( not boiled) Violet III. The mitochondrion Experiment (dry lab) Match the descriptions to the labels below. a. CO 2 production b.Site for electron transport chain and ATP-synthesizing enzymes c.Surrounds the organelle d.Compartment between outer and inner membranes 1. Intermembrane space IV. Fermentation in yeast Experiment (at home lab) Record the amount of CO 2 (as none, some or most). Container # Amount of sucrose Estimate amount of CO 2 at 0 minutes Estimate amount of CO 2 at 12 hours (none, some or most) 1 1 teaspoon 0 2 10 teaspoons 0 3 0 0 QUESTIONS: We know that yeast utilizes glucose (monosaccharide) for energy and produces carbon dioxide. You added sucrose (disaccharide) to your yeast solution. Understanding the structure of sucrose, explain why your yeast solutions produce carbon dioxide? Which cup has the maximum CO 2 production? Why? Get a 10 % discount on an order above $ 100 Use the following coupon code : NURSING10

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