[SOLVED] Kidney Cancer

Provide a 19 pages analysis while answering the following question: Characterization of Auto-Antigens as Biomarkers in Kidney Cancer. Prepare this assignment according to the guidelines found in the APA Style Guide. An abstract is required. Furthermore, the serological Expression Cloning i.e. SEREX is used to diagnose a tumor in humans causing elevated IgG antibodies.In the clear cell kidney cancer cell Caki-1, TROVE2 protein is described in isoform. In order to start this experiment Caki-1, cells are cultured under restricted conditions such as typical growth and medium standard on average supplemented 0.02% DMSO for 72 hours. Now after 24 hours, 48 hours, and 72 hours the protein is extracted while performing western blot by using monoclonal antibody elevation alongside N-terminal peptide of the human TROVE2 protein.Well 3 1.06×10^5 cells/mL – 2.01×10^4 cells/mL 20% viability. Not an obvious phenotype to indicate this level of cell death. Transfection may have work, but not sure. No protein samples prepared through Phenotypes.In the phenotypes, the difference is not observed after passing 24 hours and 48 hours. In well 3 the positive control is seen after passing 48 hours. But this is present less than the controls and the difference is calculated as 20%. In order to release each well, 1ml of TrypPLE is used at 48 hours. After completing this process 1ml of media is added and 2ml cells are removed from the tubes. The cell count is done after finishing all the steps.The gel is run at 25?g of well 2, 3, 4, 5, and 6 RIPA is extracted at 4-20% TGX gel 300v. The TBT is transferred by using a brand new machine and mixed weight settings are used. Probed overnight along with BlotCycler recycling 12.5mL of clarified antibody aliquot with added anti-Trove2 (mouse monoclonal ab58004 1/2000) and anti-SSB (Rabbit monoclonal ab124932, 1/2000). Secondary’s ab96882 and ab96884 Antibodies and Antisera.The growth media named McCoy’s 5a that is altered on an average basis and enhanced along with fetal&nbsp.bovine serum (FBS). The FBS is concentrated to 10% and x1 Gibco X100 Antibiotic-Antimycotic solution is added.

 

So much stress and so little time? Take care of yourself: let us help you with your task on
[SOLVED] Kidney Cancer
Get a 20% Discount on this Paper
Get Help Now

Haven’t found relevant content or pressed for time? –Don’t worry,  you have a team of Health & Medical professionals to help you.

All you need to do is fill in the order form in the upper right corner and upload the files that are crucial for your assignment. Also, don’t forget to specify whether your assignment has to be based upon specific research, theory, method, or program. After that, we will assign the most suitable expert to complete your assignment. You can always chat with your expert and ask any questions you have during the working process.

Calculate the price
Make an order in advance and get the best price
Pages (550 words)
$0.00
*Price with a welcome 15% discount applied.
Pro tip: If you want to save more money and pay the lowest price, you need to set a more extended deadline.
We know how difficult it is to be a student these days. That's why our prices are one of the most affordable on the market, and there are no hidden fees.

Instead, we offer bonuses, discounts, and free services to make your experience outstanding.
Sign up, place your order, and leave the rest to our professional paper writers in less than 2 minutes.
step 1
Upload assignment instructions
Fill out the order form and provide paper details. You can even attach screenshots or add additional instructions later. If something is not clear or missing, the writer will contact you for clarification.
s
Get personalized services with Do My Homeworkk
One writer for all your papers
You can select one writer for all your papers. This option enhances the consistency in the quality of your assignments. Select your preferred writer from the list of writers who have handledf your previous assignments
Same paper from different writers
Are you ordering the same assignment for a friend? You can get the same paper from different writers. The goal is to produce 100% unique and original papers
Copy of sources used
Our homework writers will provide you with copies of sources used on your request. Just add the option when plaing your order
What our partners say about us
Check out the latest reviews and opinions submitted by real customers worldwide and make an informed decision.
Nursing
Good work, professional and beat the deadline. Thank you so very much
Customer 452445, August 24th, 2021
Philosophy
Thank you! Will definitely use the writer again.
Customer 452443, October 22nd, 2021
Other
Thank you! :)
Customer 452493, May 14th, 2022
Education
Looks good. Thank you!
Customer 452523, September 27th, 2022
Nursing
Excellent! Followed directions and completed a great paper
Customer 452445, August 22nd, 2021
Economics
Although the paper did not make it at the requested time, the writer did reach out to me and asked for a short extension which I was okay with.
Customer 452493, March 16th, 2022
Nursing
A well-researched paper. Delivered before the deadline as well. Extremely pleased !!
Customer 452445, August 20th, 2021
Other
Thank you!
Customer 452493, May 29th, 2022
Leadership Studies
This is great! Thanks a bunch
Customer 452485, March 1st, 2022
Business and administrative studies
Thank you so much for your help!!!!!
Customer 452519, November 9th, 2022
Chemistry
Excellent services!
Customer 452443, November 11th, 2021
Nursing
Great services.
Customer 452443, November 22nd, 2021
OUR GIFT TO YOU
15% OFF your first order
Use a coupon FIRST15 and enjoy expert help with any task at the most affordable price.
Claim my 15% OFF Order in Chat

Order your essay today and save 15% with the discount code ESSAYHELP